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Maintaining a healthy bacterial colony

6 months ago

4 min read

The major parameters affecting bacteria growth that should be considered when maintaining a healthy biofilter are adequate surface area and appropriate water conditions.

Surface area

Bacterial colonies will thrive on any material, such as plant roots, along fish tank walls and inside each grow pipe. The total available area available for these bacteria will determine how much ammonia they are able to metabolize. Depending on the fish biomass and system design, the plant roots and tank walls can provide adequate area. Systems with high fish stocking density require a separate biofiltration component where a material with a high surface area is contained, such as inert grow media - gravel, tuff or expanded clay (Figure 2.7).

Water pH

The pH is how acidic or basic the water is. The pH level of the water has an impact on the biological activity of the nitrifying bacteria and their ability to convert ammonia and nitrite (Figure 2.8). The ranges for the two nitrifying groups below have been identified as ideal, yet the literature on bacteria growth also suggests a much larger tolerance range (6-8.5) because of the ability of bacteria to adapt to their surroundings.

Nitrifying bacteria Optimal pH
Nitrosomonas spp. 7.2-7.8
Nitrobacter spp. 7.2-8.2

However, for aquaponics, a more appropriate pH range is 6-7 because this range is better for the plants and fish (Chapter 3 discusses the compromise on water quality parameters). Moreover, a loss of bacterial efficiency can be offset by having more bacteria, thus biofilters should be sized accordingly.

Water temperature

Water temperature is an important parameter for bacteria, and for aquaponics in general. The ideal temperature range for bacteria growth and productivity is 17 -34 °C. If the water temperature drops below 17 °C, bacteria productivity will decrease. Below 10 °C, productivity can be reduced by 50 percent or more. Low temperatures have major impacts on unit management during winter (see Chapter 8).

Dissolved oxygen

Nitrifying bacteria need an adequate level of dissolved oxygen (DO) in the water at all times in order to maintain high levels of productivity.

Nitrification is an oxidative reaction, where oxygen is used as a reagent; without oxygen, the reaction stops. Optimum levels of DO are 4 -8 mg/litre. Nitrification will decrease if DO concentrations drop below 2.0 mg/ litre. Moreover, without sufficient DO concentrations, another type of bacteria can grow, one that will convert the valuable nitrates back into unusable molecular nitrogen in an anaerobic process known as denitrification.

Ultraviolet light

Nitrifying bacteria are photosensitive organisms, meaning that ultraviolet (UV) light from the sun is a threat. This is particularly the case during the initial formation of the bacteria colonies when a new aquaponic system is set up. Once the bacteria have colonized a surface (3 -5 days), UV light poses no major problem. A simple way to remove this threat is to cover the fish tank and filtration components with UV protective material while making sure no water in the hydroponic component is exposed to the sun, at least until the bacteria colonies are fully formed.

Nitrifying bacteria will grow on material with a high surface area (Figure 2.9), sheltered using UV protective material, and under appropriate water conditions (Table 2.1).

A picture containing food, fruit Description automatically generated

Water quality tolerance ranges for nitrifying bacteria
Temperature (°C) pH Ammonia (mg/litre) Nitrite (mg/litre) Nitrate (mg/litre) DO (mg/litre)
Tolerance Range 17 -34 6 -8.5 < 3 < 3 < 400 4 -8

Source: Food and Agriculture Organization of the United Nations, 2014, Christopher Somerville, Moti Cohen, Edoardo Pantanella, Austin Stankus and Alessandro Lovatelli, Small-scale aquaponic food production, http://www.fao.org/3/a-i4021e.pdf. Reproduced with permission.

Food and Agriculture Organization of the United Nations


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